ONI Nanoimager Workflow & Protocols w/ONI Access Form

1. 1. Protocol Flow Chart - Graphical instruction form for imaging EV’s
   2. List of compatible dyes - this is not exhaustive
   3. General volumes for staining in other dishes
   4. CODI Access Request Form
   5. Extracellular Vesicle (EV) Staining on a Blank Chip
   6. ONI STORM Imaging Buffer Preparation Protocol
   7. AutoEV imaging and CODI analysis protocol
   8. Manual sample preparation protocol
   9. Pan-EV, Tetraspanin Trio, and user-defined proteindetection

Workflow Integration and Data Compatibility

• Designed to align with established high-resolution imaging workflows within the HRIF.
• Compatible with commonly used single-molecule and super-resolution data analysis pipelines.
• Facilitates research continuity by supporting existing experimental designs and analytical approaches used by UAB investigators.

Overall, the ONI Nanoimager provides a proprietary integration of nanoscale resolution, dual-color TIRF microscopy, and quantitative single-molecule analysis, directly enabling UAB researchers to perform precise, high-impact investigations at the molecular scale.

For more information about gaining access to the Nanoimager, please contact Shawn Williams at 205-447-0451 or email at This email address is being protected from spambots. You need JavaScript enabled to view it.

You can access ONI’s resource library for more information about the instrument and the CODI analysis software here: https://oni.bio/resources/resource-library/